![]() Right-click on your 2D spectrum and select Properties (near the bottom). Click on 2D (at the left) and select Red-Blue for Palette, Contour for Plotting method, 10 for Number of Positive Contours, 10 for Number of Negative Contours, and for Scaling and 8.0 for Line Width. ![]() You will want to have a 1D spectrum corresponding to the F 2 dimension nucleus displayed along the top of the 2D rectangle, and another corresponding to the F 1 dimension nucleus displayed along the left side. MestReNova calls these 1D spectra Traces. ![]() For a heteronuclear inverse (HSQC or HMBC) spectrum, you will want a 1 H spectrum in the F 2 dimension and a 13 C spectrum for the F 1 dimension. For a homonuclear (COSY, ROESY, NOESY, TOCSY) spectrum you will want a 1 H spectrum in both dimensions.ΔΆ Very Important: First, set the reference for the 1D spectra before using them as traces. Reference the 1 H spectrum using TMS or the residual solvent (CHCl 3, d 5 -DMSO, etc.) peak, and reference the 13 C spectrum using the solvent peak (CDCl 3, d 6 -DMSO, etc.). ![]()
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